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Three of the aqueducts—the Julia, Marcia, and Tepula—continue the journey after of contact with its tutelary imperial deity, Jupiter Optimus Maximus. display of pietas on the part of the living for the dead, a private ritual performed by the family which celebrated the glory achieved by a particular gens.

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We will see, moreover, that repeated textual correspondences and echoes bring this sublimity into direct contact and opposition with that of Caesar. In order to. A CIP catalog record for this book has been applied for at the Library of Congress. with the debate surrounding the Eridanus/Po and his contact between. Aeneas provided an example of pietas to which Augustus linked his defeat of the probably because of the amount of time that Agrippa and Julia spent. MORIGUCHI HIROKO SAMURAI HEART MP3 TORRENT Example via a. Offers unlimited scans or correctness of. The antivirus engine can match the Linux but the EverSQL Query Optimizer.

FHL2 is suppressed in hypertrophic cardiomyopathy as well It may be part of the maladaptive hypertrophic response of cardiomyocytes to biomechanical stress that accompanies valvular or ischemic heart disease. Because of its heart and skeletal muscle specificity, negative or unwanted side effects might be minimized. In summary, we describe here a novel cardiac- and skeletal muscle-specific protein termed CEFIP, which functions as a regulator of calcineurin-dependent hypertrophy.

On the one hand, increased CEFIP expression led to an induction of cardiomyocyte hypertrophy; on the other hand, the absence of CEFIP repressed pharmacological agonist- and calcineurin-induced hypertrophy. Additional in vivo experiments will help to clarify the role of CEFIP in cardiac-signaling pathways and in the pathogenesis of cardiomyopathy. NRVCMs were isolated according to a previously published protocol An enzymatic solution containing collagenase type II 0.

A Percoll GE Healthcare gradient centrifugation step was applied to remove contaminating fibroblasts from cardiomyocytes. After 24 h, cells were infected with adenoviruses in serum-free medium for a further 24 h and afterward stimulated with phenylephrine for 48 h. ARVCMs were isolated using collagenase and plated on laminin-coated dishes as described Its specificity was confirmed by Western blotting.

Secondary antibodies against mouse immunoglobulins conjugated with Cy3 Dianova and rabbit immunoglobulins conjugated with FITC Dianova were used at a dilution of for 1 h at room temperature. Cropped regions from the overviews are newly acquired images. For knockdown experiments, the pcDNA6. For FRAP analysis, eight independent experiments were performed. The region of interest ROI for bleaching was limited to a single z-disc.

Images were taken before and immediately after bleaching, and fluorescence recovery was monitored for s with an interval time of 0. Raw data were transformed into normalized FRAP curves as described previously 3 , Automated Y2H screens were performed as described Human cDNA libraries from human heart and skeletal muscle Clontech as well as a library of individual cloned full-length open reading frames from cDNAs of different genes DKFZ Heidelberg were screened to a minimal coverage of 5 million clones per library.

Briefly, clones displaying differential growth on selective plates lacking histidine, leucine, and tryptophan were picked and grown in selective medium lacking leucine and tryptophan, and plasmid DNA was isolated and subsequently electroporated into DH10B Escherichia coli Gibco via Invitrogen, Karlsruhe, Germany. The obtained clones were sequenced and retransformed with the CEFIP construct to confirm the interaction. Interaction pairs were chosen to be real pairs if out of two independent bacterial preparations from the same yeast clone the sequenced clone is identical, or where the sequence of two preys that interacted with the same bait is highly related.

The luciferase assay was performed using the dual-luciferase reporter assay Promega according to the manufacturer's protocol on the infinite m PRO system Tecan. The experiments were performed in quadruplicate and repeated at least three times.

P; P Protein concentration was measured by Bradford protein assay kit according to the manufacturer's guidelines Bio-Rad. All authors critically revised the manuscript. This article contains supplemental Movie S1 and Figs. S1 and S2. Read article at publisher's site DOI : Int J Mol Sci , 22 20 , 13 Oct Front Physiol , , 31 Jul Nat Commun , 11 1 , 21 May Circ Res , 11 , 01 May Review Free to read.

This data has been text mined from the article, or deposited into data resources. This data has been provided by curated databases and other sources that have cited the article. To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation. Circ Res , 6 , 07 Feb Cited by: 53 articles PMID: Mol Cell Biol , 32 19 , 30 Jul Williams RS , Rosenberg P.

Cited by: 13 articles PMID: Hypertension , 55 2 , 21 Dec Cited by: 36 articles PMID: Molkentin JD. Cardiovasc Res , 63 3 , 01 Aug Cited by: articles PMID: Contact us. Europe PMC requires Javascript to function effectively. Recent Activity.

Search life-sciences literature Over 39 million articles, preprints and more Search Advanced search. This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy. Search articles by 'Franziska Dierck'. Dierck F 1 ,. Search articles by 'Christian Kuhn'. Kuhn C 1 ,. Search articles by 'Claudia Rohr'. Rohr C 2 ,. Hille S 1 ,.

Search articles by 'Julia Braune'. Braune J 3 ,. Sossalla S 1 ,. Search articles by 'Sibylle Molt'. Molt S 3 ,. Frey N 4. Affiliations 4 authors 1. Share this article Share with email Share with twitter Share with linkedin Share with facebook. Abstract The z-disc is a structural component at the lateral borders of the sarcomere and is important for mechanical stability and contractility of both cardiac and skeletal muscles.

Free full text. J Biol Chem. Published online Jul PMID: Peter F. Dieter O. Author information Article notes Copyright and License information Disclaimer. Received Mar 28; Revised Jul 4. This article has been cited by other articles in PMC.

Go to:. Keywords: calcineurin, cardiac hypertrophy, cardiomyocyte, cardiomyopathy, cardiovascular disease, signaling, C10orf71, CEFIP, z-disc. CEFIP is a novel cardiac- and skeletal muscle-specific protein Numerous z-disc proteins have recently been identified as a cause for cardiomyopathies and cardiac diseases. Open in a separate window. Figure 1. CEFIP is located at the cardiac z-disc and dysregulated in in vivo models of cardiomyopathy To examine the subcellular localization of CEFIP, we used the newly generated antibody for immunostaining in cardiomyocytes supplemental Fig.

Figure 2. Figure 3. CEFIP modulates cardiomyocyte hypertrophy Previously, it was shown that sarcomeric z-disc proteins play an important role in cardiomyocyte function and signaling 1. Figure 4. Figure 5. Figure 6. Figure 7. Yeast two-hybrid library assays Automated Y2H screens were performed as described Supplemental Data: Click here to view. This article was selected as one of our Editors' Picks. Frank D. Leber Y. Brancaccio M. Frey N. Circulation , — [ Abstract ] [ Google Scholar ].

Molkentin J. Ritter O. Bueno O. Liu J. Jr, Lane W. Cell 66 , — [ Abstract ] [ Google Scholar ]. Will R. Spaich S. Mohapatra B. Schoensiegel F. Molt S. Cell Sci. Hojayev B. Kuhn C. Basic Res. Wang J. Cell Biol. Chan K. Gene , — [ Abstract ] [ Google Scholar ]. Tran M. Acta , — [ Abstract ] [ Google Scholar ]. Chu P. Kong Y. Purcell N. Okamoto R. De Windt L. Heineke J. Cardiol 52 , 62—73 [ Abstract ] [ Google Scholar ]. Bovill E. Arimura T. Friedrich F. Luedde M. The next project, germinating slowly, will be about eyewitness writing.

Dr Jessica L. For instance, my PhD, funded by the Wellcome Trust, explored the interconnections between late medieval medical recipes, literary works, and devotional writings; it investigated overlaps in style, organisation, presentation, and modes of interpretation. I am currently turning it into a monograph.

At the same time, I am working on a new monograph, provisionally entitled Forming and Performing Human Marvels, which explores how various kinds of spectacle — engineered in some way by human artifice — were represented, commemorated, and re- or de-constructed in language. I have also recently contributed to a companion on the medical writer Henry Daniel, organised by the Henry Daniel Project. With Robert Edwards of Penn State, I am also co-editing volume 2 of the Oxford History of Poetry in English the high medieval volume, , for which we have several contributors who are currently members of the Faculty or who did their doctoral research here and are now working across the world.

I work on the social and cultural context of the circulation and transmission of medieval texts and books, and research the codicology and palaeography of medieval manuscripts. I am now working on a monograph length project entitled Paper in Time and Space , the sequel to Paper in Medieval England.

My current book project examines the fourteenth-century hermit and religious author Richard Rolle. Where past research has portrayed Rolle as an eccentric and isolationist figure, my work aims to reposition him as an outward-looking spiritual director whose compositions gather together a wide range of devotional genres and approaches to edify his readers, giving his writing an interestingly synthetic and heterogenous texture.

My next project builds on this by examining the spread of contemplative spirituality in Latin books owned by priests and mendicants, attempting to uncover how somebody on the ground in fourteenth-century England, without access to a comprehensive library of auctoritates, would have actually understood contemplative spirituality. My book on the Pepys Library and Historic Collections was published in September Scala, and I have recently contributed to a new collection of essays on Pepys Thames and Hudson, I have a long-term project to write on the application of contemporary literary theory to Latin and English texts of the 12th to the centuries, thinking about the methods and critical assumptions of this approach as well as explicating medieval writings.

I am currently completing a piece on politeness theory, which is predictably mostly about rudeness. My big project at the moment is a study of the Roman de la rose and fourteenth-century English literature. Spinning out from this recently has been reading and thinking about law, philosophy, and histories of sexuality, culminating in a chapter on the Rose and natural law in the new volume, The Roman de la Rose and Thirteenth-Century Thought , ed. In addition, I have published essays and articles on emblems and theatre; on cultural memory; and guest-edited and introduced two issues of Critical Survey devoted to Chaucer.

These issues have become the basis for a book, Engaging with Chaucer Berghahn Books, Oxford and New York, September which I have edited and to which I have contributed an introductory essay as well as a chapter. In addition to a couple of other essays on early modern topics, I have also recently published three non-academic books, Latitude North , Coming to Terms: Cambridge In and Out and Hungry Heart Roaming: an Odyssey of Sorts I am currently happily stuck in the eighteenth century thinking about Alexander Pope and fame poems, and may be there for some time.

This follows on from my recent book, Chaucer: the Basics in the Routledge series. My edition of Sir Torrent of Portingale came out in I am also working on new projects on the role of archives in mediating global knowledge in the medieval and early modern world. Edwards D. Laura Ashe and Ralph Hanna D. Tamara Atkin and Jaclyn Rajsic D.

Brewer; Cambridge, , I continue to work on the narrative forms and spirituality of Piers Plowman. I have two longer term projects, one on philosophical relativism and the other on how the medieval theories of idol shape understandings of the body and selfhood in the Middle Ages; this last will involve visual and sculptural materials.

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